FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_23.35200000038c3b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_23.35200000038c3b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1583671
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20520.12957236698784028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCG1104.1922794E-715.00328425
TATTAGG4400.013.6350432
TAGGTAG2750.013.6346135
CCTAGCG954.1790135E-412.63514330
GGTAGTC2900.012.4121997
TAGTCAG2307.2759576E-1212.3901049
GACCGCT1101.0446303E-412.2711527
GTATTAG5300.011.8867671
TCTATAC4050.011.850333
ATTAGGT3800.011.4458993
GTACTAG2659.094947E-1211.3207311
ACCGCTC950.006004033611.0512138
TTAGGTA3400.011.0279974
GTCCTGC1000.00877967510.4999781
GCCGTCT1000.00878584110.49898322
AGACCGC1000.00878789810.49865156
ACCTGTG1150.002077711310.4334437
GTATTAA4750.010.421031
CGATCAC1304.905178E-410.387217524
CGGGTCC1451.16446245E-410.34709425
Produced by FastQC (version 0.11.2)