FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_22.35200000038bf6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_22.35200000038bf6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745029
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12370.16603380539549467No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCCCG400.006516051518.7461819
TAGGTAG1151.1277734E-1018.2571535
GGTAGTC1303.45608E-1117.3041697
CTTACAC550.00210356916.3603063
TATTAGG1801.8189894E-1214.9979522
TAGTCAG1156.9501766E-714.3449069
TTAGGTA1802.1827873E-1114.1637834
GTATTAG2700.012.7777491
GTATAAA2950.012.7118361
AGTCAGA1302.7609804E-612.68972310
ACACACT1551.133285E-712.5780846
ATTATAC2650.012.45029453
GTGTAGG900.004004888211.666641
TATACTG3200.011.2477095
ATTAGGT2155.4042175E-911.1605183
GGTATAG1355.6411765E-511.1110871
CTGTACG950.006002336711.0503839
TACGGTG950.006002336711.0503837
AGTATGC950.006002336711.0503838
CTAGTAC1653.2734697E-610.906873
Produced by FastQC (version 0.11.2)