FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_21.35200000038bb2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_21.35200000038bb2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125013
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTT200.005122568530.0088027
TACTATG402.4456158E-422.5066017
CCTTTGT504.5503144E-521.0061638
CCTAATA504.564383E-520.997762
CTATGCA455.418481E-420.0058699
ACCTAAT455.418481E-420.0058691
ACTATGC500.001100543918.0052838
TATGCAA500.001100543918.00528310
GTATACA500.001100543918.0052831
TAATACT703.0795305E-517.141034
AATGTAA550.00208906616.3618918
CTAATAC653.4088452E-416.1521223
AAATGTA653.4088452E-416.15212217
ATACTAT705.970355E-415.0044026
AAAATGT705.988533E-414.99840116
AATACTA851.6166053E-414.1161415
ATCACAC650.00634865913.84467729
CAAAAAT1450.00132212379.30935214
Produced by FastQC (version 0.11.2)