FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_20.35200000038b7f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_20.35200000038b7f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338852
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6600.19477530013103067No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGTG300.001223012825.00615325
TTAGGTA455.462878E-419.9960634
CGGCCTC400.00648647618.75738327
CGGGTGG400.006492009418.75461426
ACTATAG400.006503087518.7490771
GATCGGG400.006503087518.74907723
GCGAAGG400.006503087518.74907711
GCCGACT400.006503087518.74907713
CCGACTA400.006503087518.74907714
AGGGATC400.006503087518.74907720
TACCTGC400.006508632618.746316
CCCGTCT601.8614804E-417.4991412
TATTAGG805.1610987E-616.871682
TATAATG805.1610987E-616.871682
ATTAGGT755.6115532E-515.9968523
CTATTAC600.003747872314.9992621
GCCCGTC600.003747872314.99926211
TGCCGAC600.003747872314.99926212
CAGCGTG600.003751555214.9970489
GCCGCCC706.0302985E-414.9970488
Produced by FastQC (version 0.11.2)