FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_19.35200000038b3c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_19.35200000038b3c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1678283
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25400.15134515454187403No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCACG400.006512535318.7495911
GAGCCGT755.624382E-515.9996516
GTATTAG5100.014.1177591
ACCTCGC851.634455E-414.1173388
CGATCAC1752.0918378E-1013.71521324
CGGGGTC902.653575E-413.33304113
TAGACGG1352.9010153E-713.3326445
TAGACTG1507.3931005E-812.9993295
TAGTCAG2350.012.7656799
TAGGTAG2600.012.6916525
GTCTAAG2102.8921932E-1012.1429541
GTCCTAA2257.094059E-1112.0000951
CTATGCA3900.011.9228169
TACGGAC1901.0437361E-811.84184613
GTATAGA5600.011.7858091
GGTAGTC2553.6379788E-1211.7644487
CCCACGC1151.5782354E-411.739923529
CCGCCGT1151.5782354E-411.739923524
CTCGGCC900.0040081611.66675912
CTAATAC5750.011.4776693
Produced by FastQC (version 0.11.2)