FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_18.35200000038af7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_18.35200000038af7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1997827
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36500.18269850192233864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTC1507.367271E-813.00316726
GCCGGAC954.1935995E-412.6302311
CTAATAC6300.011.9028953
CTAGACA2650.011.8849284
CCGCCTA1151.5814563E-411.7375839
CTATGCA3900.011.5369419
TAGACTG1703.7740392E-711.4687885
TAGGACA2853.6379788E-1211.0508984
TATACCG3700.010.9442295
ACTGTGC2351.879016E-910.8496338
TAGCTCG1253.4286955E-410.7983065
CTAGGCA2104.5785782E-810.71287310
TAGGTAG2802.7284841E-1110.7126055
TATACTG8050.010.6194525
CACCGCG1704.679827E-610.58842923
ACTATGC6900.010.4334078
ACGGCCA1150.002078179710.4334078
TAATACT9200.010.43314554
GTGTAAG3900.010.3842861
ACAGCGC1602.780711E-510.3111418
Produced by FastQC (version 0.11.2)