FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_17.35200000038aa7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_17.35200000038aa7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1497707
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15360.10255677512357222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG5350.014.8592341
TAGGTAG3050.014.75155455
TAGTCAG2950.014.2348339
CGTGAGC1201.1234079E-613.74946412
GGTAGTC3300.013.6340117
ACTATGC5300.013.0166238
ACGTGAG1404.5152046E-712.85578311
TATTAGG5500.012.8159692
AGTCAGA3550.012.25181610
GGCTACC1354.2010124E-612.22460426
CTAGGAC900.00401322211.6646553
CTAATAC5450.011.5576393
TTAGGTA4800.011.2480594
GCTGGGT1355.6482728E-511.11142117
CTATGCA4050.011.1091959
GTCAGAT3800.011.05146311
TGGCCAG950.006005748611.0507245
ACAGCGC950.006005748611.0507248
CGTACAC1253.4281108E-410.7981373
CTTACAC3350.010.7444153
Produced by FastQC (version 0.11.2)