FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_16.35200000038a63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_16.35200000038a63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1666183
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27590.16558805365317014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG2800.015.5337875
ACGCGCA901.5838064E-514.99858959
TGTCCCG600.00375796414.998145
GGCGCCG750.001016928714.00246525
GGTAGTC3000.013.9986837
GTATTAG4850.013.9170521
CCCCGGC1004.274128E-513.49954123
CGCGCAT1151.0947078E-513.04225310
TAGACTG1753.079549E-912.8555475
GTCTAGA1551.13404894E-712.5802171
ACCGCTC850.002614955212.351788
GACGCGC1006.45675E-411.9988728
TAGTCAG3050.011.8021699
CTCGGTC1151.5805976E-411.7380289
ATGCGGT1151.5805976E-411.7380286
TACGTCC900.00401209211.665224
CGGTCAG1202.3473585E-411.24894211
TGCGCGA950.0060027211.05159310
TAGGGGT1000.00878793610.4986984
CAGCGCA1150.002077214710.4338029
Produced by FastQC (version 0.11.2)