FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_15.35200000038a20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_15.35200000038a20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1692799
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22580.1333885476066562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG4500.012.9992061
TAGGTAG2700.012.2207555
ACTATGC4600.012.0641258
GGTAGTC2701.2732926E-1111.1101057
TATACTG6350.010.8648375
GCCGCGT1253.4166628E-410.80221325
TACACCG1253.4269484E-410.7987025
TTAGGTA4050.010.73944954
GTGTAAG2802.7284841E-1110.7136321
CGAGGCC1000.00878585410.49904921
CAGTGCG1000.00878585410.4990499
AGGACCG1000.00878777810.4987395
CGCTCCT1304.9036095E-410.38766426
CAGGACC1451.16857496E-410.3435854
TCACACA3051.2732926E-1110.33090130
ATCACAC3101.8189894E-1110.16427229
ACCTAAT5650.010.087881
TAGGACA2403.0067895E-89.9987994
CTAATAC6450.09.9987983
TACAGCG1851.282302E-59.7288487
Produced by FastQC (version 0.11.2)