FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_14.352000000389eb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_14.352000000389eb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1549587
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24720.15952637702820172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCA1002.5755653E-614.9996299
GACGCGC600.003755324814.9996298
CTAGTAC1601.71578E-712.1864113
GTACAAG3101.8189894E-1210.6455841
CGGGGAC1000.00877260510.50109725
CCGCGTT1150.002073093610.43620928
AGCTTAG1200.002953875610.0003981
GATTCGG1356.963758E-49.99910722
CTTAGAC2105.256388E-79.9991073
CTAGACC1552.2893609E-49.6765554
GTATTAG4200.09.6432411
TAGGTAG2352.3657776E-79.5736135
TAGACAG2209.925625E-79.5446025
TAATACT6300.09.2848854
CGTACCG1300.00572170649.23083914
TTATCCT4400.09.2037244
GTATAAT7350.09.1840391
CCTACAC1801.01048005E-49.1658493
CTAATAC3953.6379788E-129.1131113
TACACAG2803.3125616E-89.106335
Produced by FastQC (version 0.11.2)