FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_13.352000000389a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_13.352000000389a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1849271
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33000.17844869681079734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG2650.011.8856895
ACAGTCC1652.558627E-711.8177238
CGCGCCT900.00400820311.66684622
GTATTAG5350.011.2148221
TAGACAG3150.010.95136455
TACACAG2251.1024895E-810.6656775
TGTACCG1851.1225038E-610.5395615
CTGTCCG1000.00878310.4995929
GAGCGCC1000.00878310.49959219
CCGTCGC1150.002076446310.4343789
TCTTATA5700.09.9993422
TAGGTAG2403.0064257E-89.9990715
TATACAG8350.09.8793225
GGTAGTC2905.693437E-109.826947
TAGTCAG2454.1334715E-89.7955389
TATACCG3850.09.7393555
ACCGTCG1552.2887366E-49.6770458
GAGGGCG1409.7187056E-49.6424849
CCTGGAC1409.7235205E-49.6419623
ACTATGC4850.09.5872578
Produced by FastQC (version 0.11.2)