FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_96.35100000039e6f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_96.35100000039e6f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677103
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT41190.6083269458265581No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC29990.4429163657523301No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC29540.43627040494577635No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC23830.3519405467115047No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG23120.3414546974389421No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT21580.3187107426787357No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC21520.3178246145711952No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG20880.30837258142409646No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC18770.2772104096422553No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT18740.2767673455884851No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT15370.22699648354829327No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG15240.2250765393152888No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG15100.22300890706436097No Hit
GGATGTGATGAGCCGACATCGAGGTGCCAAACACCG14060.20764935320032551No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG13910.20543403293147425No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG13500.1993788241966141No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT13440.1984926960890736No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC12880.1902221670853622No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC12750.18830222285235776No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC12430.18357620627880838No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT11850.17501030123925015No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC11850.17501030123925015No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT11710.1729426689883223No Hit
GGAAACCTTGGTCTTTCGGCGTGGGTGTTTTTCACA11400.16836434043269635No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC10760.1589123072855976No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT10660.15743542710636343No Hit
TGCGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGA10240.1512325303535799No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC10170.15019871422811595No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT10140.1497556501743457No Hit
CCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGGT10010.14783570594134127No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA9810.14488194558287293No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC9500.14030361702724697No Hit
GAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGT9380.13853136081216597No Hit
ATCGAGGACAGTGTATGGTGGGTAGTTTGACTGGGG9280.13705448063293177No Hit
ATTCCCAGCGACTAGACGCCCTATTAAGACTCGCTT9090.13424840829238682No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG8930.13188540000561216No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG8870.13099927189807165No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA8610.12715938343206276No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA8570.1265686313603691No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT8510.1256825032528286No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT8420.12435331109151783No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT8360.12346718298397732No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC8290.1224333668585134No Hit
GTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTA8190.12095648667927923No Hit
CTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTC8000.11815041433873429No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG7950.1174119742491172No Hit
CCGTTATCCGGATCGAGGACAGTGTATGGTGGGTAG7930.11711659821327036No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT7840.1157874060519596No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA7820.11549203001611276No Hit
GGCGTAAAGCGCGCGTAGGTGGTTTGTTAAGTTGGA7620.1125382696576444No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC7590.11209520560387415No Hit
GTATAATGGGTCAGCGACTTATTTTCAGTGGCGAGC7480.11047063740671655No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC7460.11017526137086972No Hit
CCTAAAGAGTAACGGAGGAGTACGAAGGTGCGCTCA7430.10973219731709946No Hit
CTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC7370.10884606920955897No Hit
TTCCTGTACTTCTGGTTATTGCGATGGAGGGACGGA7360.10869838119163555No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC7350.10855069317371212No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT7350.10855069317371212No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC7280.1075168770482482No Hit
GCTAACTTTTCAAGGCCGAGAGCTGATGACGAGTGT7230.1067784369586311No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC7080.10456311668977983No Hit
ATCCGGATCGAGGACAGTGTATGGTGGGTAGTTTGA7040.10397236461808616No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG6950.1026431724567754No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG6950.1026431724567754No Hit
GAGTTAGCCGGTGCTTATTCTGTCGGTAACGTCAAA6880.10160935633131149No Hit
CCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA6790.10028016417000071No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATTGG200.005144916530.00103220
ACTCGGT1700.023.8225923
TATGCGC4450.023.59457611
ACCGTAT7550.023.045457
CCACCGT7550.023.045455
ATCCACC8100.022.4065253
CCGTATG7850.022.3558088
CATCCAC8400.021.7848552
TATACAC350.003005578921.4277293
GTATGCG8300.021.32446110
ATGCGCA5150.020.97004712
CGTATGC8400.020.8920359
TATGCGT3450.020.86874411
ATCGTTA2450.020.81550611
CTCGGTC1950.020.7684144
CACCGTA8450.020.5909066
GAGCCAT3650.020.5547261
TCCACCG8900.020.3924564
CATTCGT2400.020.0006921
TTCCTAG455.464826E-419.9992123
Produced by FastQC (version 0.11.2)