FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_95.35100000039e39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_95.35100000039e39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931556
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT74730.8022062012374993No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC47320.5079673149010903No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC37960.40749026360197343No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG32580.34973742856038714No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT26650.2860804932822074No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT26520.2846849786808308No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG26420.2836115059105411No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC26360.28296742224836724No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC25790.2768486274577159No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG25660.2754531128563393No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG24770.2658992052007609No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC23620.2535542683424292No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG22470.2412093314840976No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT22000.23616400946373595No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG21270.22832765824062107No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT20430.2193104869701875No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC20110.21587537410526045No Hit
GGATGTGATGAGCCGACATCGAGGTGCCAAACACCG19430.20857575926729044No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA18640.20009532438200173No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC18330.19676755879410365No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC18240.1958014333008429No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT17660.18957529123316258No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC17030.1828124127803374No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT16900.18141689817896078No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT16830.180665467239758No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC15680.16832053038142633No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG15180.1629531665299778No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC15000.16102091554345632No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT14840.1593033591109928No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT14360.1541506898136022No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT14310.15361395342845732No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT14130.15168170244193585No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG13280.14255718389447333No Hit
ATCGAGGACAGTGTATGGTGGGTAGTTTGACTGGGG13280.14255718389447333No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG12900.13847798736737243No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT12820.13761920915114068No Hit
CCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGGT12800.13740451459708275No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA12760.13697512548896684No Hit
GGAAACCTTGGTCTTTCGGCGTGGGTGTTTTTCACA12670.1360089999957061No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC12670.1360089999957061No Hit
TGCGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGA12630.1355796108875902No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA12560.13482817994838742No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC12440.13354001262403978No Hit
CTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTC12160.1305342888672286No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT12100.12989020520505476No Hit
CCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA11990.12870938515773608No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG11900.12774325966447536No Hit
GGTTCGCCTCTTGCACCTATGTATTCAGTACAAGAT11840.12709917600230153No Hit
CCTAAAGAGTAACGGAGGAGTACGAAGGTGCGCTCA11430.12269793764411371No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC11400.1223758958130268No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA11390.12226854853599783No Hit
TATCTAGGTAGCGCCTCATGTATCACTGTAGGGGGT11290.12119507576570812No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG11170.11990690844136047No Hit
CTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTG11110.11926282477918665No Hit
TTCCTGTACTTCTGGTTATTGCGATGGAGGGACGGA11110.11926282477918665No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC11090.11904813022512871No Hit
ATTCCCAGCGACTAGACGCCCTATTAAGACTCGCTT11060.11872608839404179No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCG10710.11496893369802781No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC10360.11121177900201384No Hit
GAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGT10210.10960156984657926No Hit
CGTCTTTCATCGCCTCTGACTGCCAAGGCATCCACC10190.10938687529252134No Hit
GGCTAGGCCAGCCTGGCGTTGGTTGTCCAGGTTTAA10180.10927952801549234No Hit
CCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGT10110.10852809707628958No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC9950.10681054064382602No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC9820.1054150260424494No Hit
GGCCTAGCCTTCTCCGTCCCTCCATCGCAATAACCA9820.1054150260424494No Hit
GTATAATGGGTCAGCGACTTATTTTCAGTGGCGAGC9800.10520033148839146No Hit
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACC9700.10412685871810175No Hit
CTCCTCTACCGCATCATCAAAAGATGATACCCGTAG9580.10283869139375412No Hit
TCTTTTGATGATGCGGTAGAGGAGCGTTCTGTAAGC9430.10122848223831954No Hit
CTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC9320.10004766219100086No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGGA200.00514721429.9994623
TACCCTA300.001226507824.9995525
ACCCGTG1050.022.8567353
ACCTAGA402.4655284E-422.50201228
CTCGGTC3050.022.130754
ACTCGGT2850.022.1048683
CCATGAT4500.021.9996074
CTAGATA350.003004171221.43048930
CTTTCAT2250.021.3329524
ACCTATG2250.021.33180815
CATGATC4700.021.0634525
AGTCACA4350.021.03184912
TCTTATA504.6020657E-520.9996242
ACCGTAT8500.020.9996247
CGTCTTT2150.020.9377251
GGTCCTA657.091621E-720.7710929
CCACCGT8600.020.7554425
CCGTATG8850.020.6764858
TCTGACT1900.020.52484515
GCACCTA2350.020.4240713
Produced by FastQC (version 0.11.2)