FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_94.35100000039df4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_94.35100000039df4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660865
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT51480.7789790653159118No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC23540.3561998290119767No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC19740.2986994318052855No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG19560.2959757287797054No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG18380.2781203422786802No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG14970.22652130162741255No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT14300.21638307369886436No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC14290.2162317568641099No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT14010.2119948854909853No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG14000.21184356865623083No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC13630.2062448457703162No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG13550.20503431109228057No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA13300.2012513902234193No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT12970.19625793467652244No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG12700.19217238013815227No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC12640.19126447912962558No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT11900.18006703335779622No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT11310.17113934010728363No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC11020.16675115189940456No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT10670.1614550626829988No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC10000.1513168347544506No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT9790.14813918122460715No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC9670.14632337920755373No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG9650.14602074553804484No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCG9460.14314572567771028No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC9020.13648778494851446No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA8940.13527725027047885No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT8860.13406671559244324No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA8770.13270486407965318No Hit
CGTTAGCACCCGCCGTGTGTCTCCCATGCTCGGCAC8720.13194827990588093No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT8610.13028379472358198No Hit
GGATCACAGTCTGTTTGCCGACTCCCCGAAGCTTTT8560.1295272105498097No Hit
CCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGT8540.1292245768803008No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG8220.1243824381681584No Hit
TATCTAGGTAGCGCCTCATGTATCACTGTAGGGGGT8000.12105346780356048No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG7930.11999424996027933No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT7920.11984293312552487No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC7810.11817844794322592No Hit
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACC7790.11787581427371702No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT7750.11727054693469922No Hit
CCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA7690.11636264592617251No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA7560.11439552707436465No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC7470.11303367556157459No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG7420.11227709138780236No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC7310.1106126062055034No Hit
CCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGGT7160.10834285368418663No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG7090.10728363584090547No Hit
ATATTAACCTGTTTTCCATCGACTACGCTTTTCAGC7030.10637573483237878No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC7010.10607310116286987No Hit
ATCGAGGACAGTGTATGGTGGGTAGTTTGACTGGGG6880.10410598231106202No Hit
CCTAAAGAGTAACGGAGGAGTACGAAGGTGCGCTCA6710.10153359612023637No Hit
CTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTC6700.10138227928548191No Hit
ATGTATCACTGTAGGGGGTAGAGCACTGTTTCGGCT6640.10047437827695521No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC6620.1001717446074463No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC6620.1001717446074463No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATAGC300.001226615924.9978568
CATACCC8600.021.6260533
GGTCCGA350.002999720621.4348431
CTCTTAT350.002999720621.4348431
CCATACC8750.021.255322
ACCGTAT4800.021.2481777
TACCCTT8550.021.226255
GCCATAC8700.021.2131021
CCGTATG4900.020.814548
TATGCGC3650.020.1352611
ATACCCT9250.019.7820874
CTCGGTC1450.019.6534884
CACCGTA5200.019.6137036
CCACCGT5250.019.4269055
ACCCTTG9400.019.1472936
CTGAGAG400.006510857518.74839214
CGTATGC5450.018.7139919
GTATGCG5600.018.48055810
TCTGACT1301.8189894E-1218.45995515
TGTAGGA904.2680767E-818.331762
Produced by FastQC (version 0.11.2)