FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_93.35100000039db0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_93.35100000039db0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73553
Sequences flagged as poor quality0
Sequence length36
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT3460.4704090927630416No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC2600.35348660149823935No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC2520.34261009068290893No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT2170.2950253558658382No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC1740.2365641102334371No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT1720.23384498252960448No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG1460.19849632237978054No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC1450.19713675852786428No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT1410.19169850312019904No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC1360.18490068386061753No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT1280.17402417304528708No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG1260.17130504534145446No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA1210.16450722608187293No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG1200.16314766222995664No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC1090.14819245985887727No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG1070.14547333215504465No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC1060.14411376830312836No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG1060.14411376830312836No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT1050.14275420445121206No Hit
CGTTAGCACCCGCCGTGTGTCTCCCATGCTCGGCAC1040.14139464059929574No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT1000.13595638519163053No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC970.13187769363588162No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT950.129158565932049No Hit
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACC940.1277990020801327No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC930.12643943822821638No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA920.12507987437630008No Hit
CCCATTATACAAAAGGTACGCAGTCACCCAACAAAG900.12236074667246748No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT890.12100118282055118No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC860.11692249126480224No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT840.11420336356096963No Hit
ACCAGGAACCGTACCCCAAACCGACACAGGTGGTTG810.11012467200522072No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT790.10740554430138811No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG790.10740554430138811No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG780.10604598044947182No Hit
GGTACGCAGTCACCCAACAAAGTGGGCTCCCACTGC760.10332685274563921No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC760.10332685274563921No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG750.10196728889372289No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC740.10060772504180658No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGT254.1883817E-430.011
CCGTATG750.028.08
CCATACC603.8016879E-1027.52
TACCCTT554.5638444E-927.2727265
TATGCGC505.4900738E-827.011
GTATGCG800.026.24999810
ACCGTAT800.026.2499987
GCGTACA359.85165E-525.71428514
GCCATAC659.640644E-1025.3846171
ATACCCT601.1528755E-825.04
CATCCAC900.024.9999982
CCACCGT900.024.9999985
CGTATGC851.8189894E-1224.7058839
CATACCC751.9281288E-1024.03
CACCGTA950.023.6842126
TCCACCG950.023.6842124
AAGCAGT950.023.68421220
ACCCTTG652.697925E-823.0769256
ATCCACC1000.022.53
GCGCACA603.201185E-722.514
Produced by FastQC (version 0.11.2)