FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_92.35100000039d7d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_92.35100000039d7d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133691
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT6770.5063916045208727No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT3470.2595537470734754No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC3330.2490818379696464No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC3270.24459387692514828No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC3180.23786193535840108No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG3090.2311299937916539No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG3000.2243980522249067No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG2940.21991009118040855No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC2760.20644620804691413No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT2520.1884943638689216No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC2350.17577847424284357No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT2190.16381057812418187No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG2040.15259067551293656No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG1990.14885070797585476No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG1830.1368828118571931No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT1830.1368828118571931No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG1820.13613481834977673No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC1730.12940287678302953No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA1720.12865488327561317No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC1700.12715889626078045No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT1650.12341892872369867No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC1650.12341892872369867No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA1640.12267093521628233No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC1630.12192294170886597No Hit
GGACCGAACTGTCTCACGACGTTCTAAACCCAGCTC1620.12117494820144961No Hit
TCTCTACCCAACCACCTGTGTCGGTTTGGGGTACGG1610.12042695469403326No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC1580.11818297417178418No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT1560.11668698715695147No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG1550.11593899364953511No Hit
CCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGT1540.11519100014211878No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG1520.11369501312728605No Hit
CGTTAGCACCCGCCGTGTGTCTCCCATGCTCGGCAC1520.11369501312728605No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT1450.10845905857537155No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT1410.10546708454570615No Hit
CTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC1410.10546708454570615No Hit
CTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTG1390.10397109753087343No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG1380.10322310402345707No Hit
GTTTTCCATCGACTACGCTTTTCAGCCTCGCCTTAG1370.10247511051604072No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC1350.100979123501208No Hit
ATTCCCAGCGACTAGACGCCCTATTAAGACTCGCTT1340.10023112999379166No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGGG200.00512211430.0108531
TTCTCCG200.00513150129.99962610
CTAGCCT200.00513150129.9996264
TATGCGC456.702412E-726.66633411
TAGGGAC300.001220978724.999693
CGTATGC755.149559E-921.9997259
CCGTATG755.149559E-921.9997258
GTATGCG755.149559E-921.99972510
ACCGTAT755.149559E-921.9997257
CCACCGT755.149559E-921.9997255
GCGCACA553.843168E-621.81790714
CTTAAGT350.0029926521.42830530
CCTTAAG350.0029926521.42830529
TCGCAAT350.0029926521.42830525
CAGCTAG801.083572E-820.6247439
AGGAACC455.432384E-419.999754
TTTCCCA608.162919E-619.9997521
GCACAAG608.162919E-619.9997516
ATGCGCA608.162919E-619.9997512
GAACCGT455.432384E-419.999756
Produced by FastQC (version 0.11.2)