FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_91.35100000039d3a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_91.35100000039d3a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118922
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT10870.9140444997561427No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC4340.36494509005903025No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC3760.31617362641058844No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG3590.30187854223776933No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG3310.2783336977178319No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT3170.2665612754578631No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG2830.23797110711222483No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT2660.22367602293940567No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT2590.2177898118094213No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC2550.2144262625922874No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC2310.19424496728948384No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC2260.19004053076806646No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG2250.18919964346378298No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT2100.1765863338995308No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG2080.17490455929096382No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC2070.17406367198668032No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC2050.17238189737811338No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT2010.16901834816097946No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA2000.168177460856696No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG1970.16565479894384555No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG1890.15892770050957772No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC1840.1547232639881603No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC1730.14547350364104203No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC1670.14042817981534114No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG1570.13201930677250634No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA1520.12781487025108895No Hit
GCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAA1450.12192865912110458No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC1410.11856510990397068No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC1370.11520156068683676No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT1350.11351978607826979No Hit
ATCTAGGTAGCGCCTCATGTATCACTGTAGGGGGTA1320.11099712416541936No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC1320.11099712416541936No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG1290.1084744622525689No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT1290.1084744622525689No Hit
ACCATACACTGTCCTCGATCCGGATAACGGACCTGA1290.1084744622525689No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG1270.10679268764400196No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC1250.10511091303543499No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC1240.10427002573115152No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA1240.10427002573115152No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA1230.10342913842686803No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT1210.10174736381830106No Hit
GGCTAGGCCAGCCTGGCGTTGGTTGTCCAGGTTTAA1210.10174736381830106No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT1200.10090647651401759No Hit
CTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC1200.10090647651401759No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAACC200.00512084430.00967829
ATAGTGG200.00513139529.9970596
CATACCC2050.021.9490663
ACCTAAA553.8261005E-621.8252181
TACCCTT1950.021.5363485
CAGGTTT350.002984914221.43548428
AGGTTTA350.002984914221.43548429
GCCATAC2350.021.0706231
CCATACC2150.020.9281772
ATACCCT2100.020.7122544
ACCCTTG2150.019.5329676
AGCTATC559.464218E-519.09706726
AGGAGAA559.4948686E-519.08903517
AACCAGC559.4948686E-519.08903522
CCAGCTA559.4948686E-519.08903524
GAGAACC559.4948686E-519.08903519
CCACTTA400.00646495418.75604825
TCCTCTA400.006480687318.74816115
TTTCCCA400.006480687318.74816121
TATGCGC802.452707E-718.74816111
Produced by FastQC (version 0.11.2)