FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_90.35100000039cf5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_90.35100000039cf5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71412
Sequences flagged as poor quality0
Sequence length36
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCA2160.3024701730801546No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATG1300.1820422337982412No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT200.005115365629.9999981
TCTTATA455.397922E-420.02
AGGCATG455.397922E-420.030
TAGGCAT455.397922E-420.029
TTATACA805.037662E-616.8752
CTCCGAG550.002075784116.36363612
TCCGAGC550.002075784116.36363613
CCGAGCC550.002075784116.36363614
CGAGACT653.3805167E-416.15384723
CTAGGCA653.3805167E-416.15384728
AGACTAG705.939184E-415.025
ATCTCCG705.939184E-415.010
TACACAT705.939184E-415.05
GACTAGG705.939184E-415.026
ACTAGGC851.5998518E-414.11764727
ACACATC750.001001621114.06
ACATCTC750.001001621114.08
TATACAC750.001001621114.03
AGCCCAC650.00630905913.84615317
CACGAGA650.00630905913.84615321
Produced by FastQC (version 0.11.2)