FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_89.35100000039cb1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_89.35100000039cb1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences701820
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGGG455.4652215E-419.9991469
CCCGGGG852.2142558E-819.41231726
CCCCTAT500.00110783518.0043621
CCGGGGC1007.2486728E-918.00051327
GCCCGGC1351.1041266E-915.55489111
GTGCGCG600.003753048414.9993599
GAGCCCG1503.0377123E-1014.9993589
CCCGGCC1351.8479113E-814.44485712
AGCCCGG1351.8495484E-814.44382710
GCCCGGG1251.1301745E-714.40041125
GGGCCCG1251.13115675E-714.399384523
AGGAGCC1504.8858055E-913.9994017
CCGGCCC1402.98096E-813.92896813
GCGGGAA650.006381579713.84654815
CCCGGAG1454.7235517E-813.44865917
GGAGCCC1454.7279173E-813.4477018
ACAGCGC800.001655821713.1244398
TACCGGC800.001655821713.1244397
CGGCCCG1507.361268E-813.0003714
GCCCGGA1551.1294833E-712.58100416
Produced by FastQC (version 0.11.2)