FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_88.35100000039c7e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_88.35100000039c7e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017076
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCCT350.003004661821.43011724
TATACTG3350.012.9840965
CGAGACT900.004004383511.66750825
CCTAGAC900.00400879911.6657873
TAGACTG1303.845436E-511.5375915
ACTGATC1355.650081E-511.1102748
GTCTAGG950.00598381711.0556031
TATTATA6150.010.9747822
ATGTGGG1100.001433338210.9082697
CGGTGTT2207.741619E-910.9082699
ACGGTGT2207.741619E-910.9082698
GTGTAAT2504.4929038E-1010.8029031
TCCTATA1551.9549125E-510.6443592
CACATCG1000.00878082210.4992085
TAAGACT1000.00878082210.4992084
TCTACAC1653.8941012E-59.9992473
TGGATGG1653.8941012E-59.9992475
CAGAGCA1200.00295520629.9992474
TACGGTG2557.18137E-99.9992467
GTACTGG1552.2793887E-49.680021
Produced by FastQC (version 0.11.2)