FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_87.35100000039c2e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_87.35100000039c2e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1210996
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGTA805.175052E-616.874499
ACCGTTC1557.765266E-913.54797658
GACCGTG902.652809E-413.3329297
GGACCGT902.652809E-413.3329296
CCGAGCC800.001656359213.12514516
ACCGTGT1056.752259E-512.8567538
CCTAAGG850.002612993812.3525673
TAGACCG1354.209718E-612.2218525
CCTTAAG1252.5660034E-512.0036021
TAGGACT900.004008203311.6663134
TCTAGAC1303.8450737E-511.5381133
TATACCG2503.8198777E-1111.3996555
TACCGTA1602.2618842E-611.249667
TGGACAG1755.474758E-711.1425195
TGGACCG950.00599827111.0522975
CTAATAC2853.6379788E-1211.0522973
TCTAGCC1253.4225814E-410.7996733
ATTATAC4250.010.5879143
GCCCTGG1150.002069715410.4379151
CCTATAC2053.771438E-710.2435923
Produced by FastQC (version 0.11.2)