FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_86.35100000039c04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_86.35100000039c04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1089448
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGTA350.003006801521.4276529
GTGCGAG500.001110491717.9992311
CGCGCAT600.003754824414.99935710
CTAATAC2550.012.3529793
CTTGCCC1006.448325E-412.0000373
GACATAC900.004002642411.6683081
TCTATAC2751.8189894E-1211.454583
GTGCTAC1059.698634E-411.4301811
CTAAGAC1459.3287E-611.3793453
TATAAGG2504.5292836E-1010.8000332
TTAACCT2104.5600245E-810.7143184
TATCCTG2802.7284841E-1110.7143185
GTAGCGC1000.00877937310.4995518
CTGTATG1150.0020753410.4343359
CTATGCA1756.6108914E-610.2852749
CTATACT2352.1609594E-810.2127964
ACTATGC2505.187758E-910.1995628
TAAACTG2803.0013325E-1010.1786025
TATACAG3650.09.8630455
CCTAATA3357.2759576E-129.8507772
Produced by FastQC (version 0.11.2)