FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_85.35100000039bcf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_85.35100000039bcf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450441
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGCT701.441942E-619.2852154
TAGAGGT600.003749847614.9996124
AGTAGGC706.027946E-414.9996123
GGGTCCC650.006379198313.84579526
TAAAGTG850.002608514612.3526225
ATTGCAC850.002608514612.3526223
ACACAAT1006.4369605E-411.999696
TCCTATA900.00400139511.6663642
GTCATAT1303.832417E-511.5381621
ATACTAC1059.692869E-411.4282756
CTTGCCA950.005988173711.0523459
AAAATCG950.005988173711.05234515
TTATACT1807.8322046E-710.8330524
TTAGTAG1150.002070985310.4345133
TATGTAC1150.002070985310.4345133
GTTATAT1150.002070985310.4345131
TCTTAGA1304.903066E-410.3843462
TTTAGTA1451.1631193E-410.344562
TATACTG1200.0029485479.9997425
TCATATA1809.205518E-69.9997412
Produced by FastQC (version 0.11.2)