FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_84.35100000039b8c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_84.35100000039b8c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736307
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGG300.001226378724.99915129
CGTGAGC455.465249E-419.9993212
GTGCGGG400.00650960318.74936330
GGTAGTC651.64508E-518.460917
ACGTGAG550.002101155216.36308111
GTCTAGG653.4212132E-416.1620791
TAGTCAG755.6180907E-515.9994569
GTATTAG1604.9112714E-1115.0076431
CATGCAC600.003753062614.999493
TAGGTAG1251.1313023E-714.3995115
CTATGCA1156.936534E-714.3473399
CCGTCGT650.00638461113.84568329
TAGAGCG800.001655847313.1245545
CCTATAT1056.711242E-512.8636951
TAGACTG850.002611388212.3525225
ATACACT2102.8921932E-1012.1424454
TACACTG1151.5774212E-411.7387315
AATACAG1859.136056E-811.3509665
TAGAACT1602.2580316E-611.2496184
TAATACT3750.011.1996194
Produced by FastQC (version 0.11.2)