FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_83.35100000039b49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_83.35100000039b49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856982
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGAC601.8655614E-417.4992853
CGCGTAT750.001017888513.99942810
CGACGGT650.00638355913.84639630
TAGGACT650.00638602713.8455894
CTATGCA1454.7326466E-813.4477269
ACTGCGT800.001656318213.1244648
GTATTAT4400.012.6145931
GTACTAG850.002609840612.3538781
ATACCGT1501.0384665E-611.999516
TACGGTG2301.05501385E-1011.738657
ATACGGT2103.7307473E-911.4281046
TAGGACA1059.7097066E-411.4281044
GTATTAA2503.8198777E-1111.4008651
ACGGTGT1859.1446054E-811.3508888
GGGAGCC950.005991113411.05347124
TGACCAC950.005996319511.0521810
TACTATG2853.6379788E-1211.052187
TATACGG2053.1974196E-810.9751625
CCCTTGA1100.001431131510.9099181
CTAATAC2351.8681021E-910.8506213
Produced by FastQC (version 0.11.2)