FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_82.35100000039b12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_82.35100000039b12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173081
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGCC200.00513701829.99797612
TCAGGCT200.00513701829.99797618
TATTAAG350.002997137121.4271262
TAGTACC455.443305E-419.9986524
TAAGACT400.00649121518.7487354
TTATACT651.633734E-518.4602954
CTTCTAA500.001105551417.9987871
TATTAGC550.002092894616.3625342
ACTATGC653.4173223E-416.1527588
CATACTG600.00373846214.9989895
ATACTAT750.00101233713.9990576
TATATAC1459.210187E-611.3785433
ATATACA1300.00568223569.2301474
Produced by FastQC (version 0.11.2)