FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_81.35100000039add.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_81.35100000039add.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368922
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACT350.00300327421.427414
CTTACAC455.458897E-419.9989153
ATAAGAC400.006504346618.7489853
ACTGTGC400.006504346618.7489858
GGTTAAG400.006504346618.7489851
GGTAGTC601.8621191E-417.4990527
TAAGACA550.002098765716.362754
CCTCTGA653.4301265E-416.1529711
CTATACT1156.91105E-714.3470484
GTATTAT1802.1827873E-1114.1658971
CCTATAC750.001016061813.9992433
TTAGGTA650.006377507513.8454044
CTACTCT650.006377507513.8454044
AACCAGC650.006377507513.8454046
TTATACA1106.8828504E-613.6356244
TCTAGAC800.00165337313.12428953
GTGTATA800.00165337313.12428951
TATAAGA1151.0887779E-513.042772
TAAAGCA1056.729057E-512.8564454
AGTACTG1056.729057E-512.8564455
Produced by FastQC (version 0.11.2)