FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_80.35100000039a9a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_80.35100000039a9a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252548
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGG651.6387543E-518.4600752
TGTACTG550.00209664716.362345
GTATTAG755.587364E-516.0019021
TACTATA706.017382E-414.9988122
AAAGCGT706.017382E-414.9988126
GTATAGA851.6238492E-414.1193261
TCTAATA851.626585E-414.1165292
GTCATAA650.006362859613.8477991
GTGTAGA650.006362859613.8477991
TACAGCG650.006371216413.8450577
CTAATAC1004.247888E-513.498933
TTCTCTA800.001651169313.12396057
TTGTACT1151.0858226E-513.0424454
TAATACT1302.7366987E-612.6913024
CATATAC954.1701383E-412.6305783
GTCATAT850.002600214212.3544091
TAGAATA1006.4225885E-411.9990494
AATACAG1006.4225885E-411.9990495
CTATACA900.00398875311.6680541
AATAATA1551.5287715E-611.6119832
Produced by FastQC (version 0.11.2)