FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_79.35100000039a57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_79.35100000039a57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699801
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAGC400.00650872818.7496438
AGACGGC653.434711E-416.153546
GGTGCGA809.745068E-514.99971428
AGCGTGT952.6327054E-514.21025511
GTGCGAC851.6322678E-414.11737829
GCCGCAC650.00638356213.8458918
TACAGGC1004.2665997E-513.49974257
TGCGACG902.6484492E-413.33403230
CTAATAC1803.274181E-1013.333083
TAATACA2051.4551915E-1113.1704814
ATTAATC800.001655518713.124753
TTAGGAC1151.0917089E-513.0432293
CAGCGTG954.184457E-412.63133810
TAAGCCT954.184457E-412.6313384
ACTATGC2307.2759576E-1212.39106758
TAGACTG850.00261087312.3527065
TCGACTC850.00261087312.35270613
GAACGTA1101.0371015E-412.2786341
ACAGGCA1354.2020292E-612.221998
CTATGCA1601.7090133E-712.1872689
Produced by FastQC (version 0.11.2)