FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_78.35100000039a13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_78.35100000039a13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2309032
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC6550.011.9083013
GGCGCCG900.00400811811.6670825
CTAATAC7050.011.489273
CGCCGCG1408.180247E-510.71443324
GTATTAT10450.010.6258371
GTGCGAC2156.455048E-810.4652629
CTCTTAA4450.010.4532031
GTATAGA5750.010.4385431
TACTATG7000.010.285417
ACTATGC6150.010.2433788
CTATACT6550.010.0762554
CAGCGTG1809.261317E-69.9994889
ATACTAT11250.09.7332566
CCTTATA4650.09.6773432
ACCGTGC1409.7217463E-49.6423648
GTATTAA6250.09.6034591
GTATATG8950.09.5565151
TAATACT10550.09.52599054
TATACTG8700.09.4826835
GTATAAA8750.09.4319691
Produced by FastQC (version 0.11.2)