FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_77.351000000399de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_77.351000000399de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1604206
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCAG1801.8189894E-1214.9991599
TTAGGAC1958.367351E-1113.076193
GTATTAG4450.012.4750991
GGTAGTC2154.292815E-1011.8597987
CTATGCA3700.011.7560989
CCTAGCG1501.35865685E-511.00109730
TAGGACT1501.3610061E-510.9993824
TAGGTAG2802.7284841E-1110.7136855
AGTCAGA2556.4028427E-1010.58764110
TAGCCTG1704.682128E-610.5876415
AGGTAGT3750.010.3994176
CGATCAC1451.1666259E-410.34521524
GTCTAAG2208.997995E-810.2298881
GTGTATA4350.010.0025571
TACCGAC1653.897089E-59.9994397
TATTAGG5400.09.7216772
AGACTGT1705.4081484E-59.7053386
CTTATAC5050.09.5044173
AGACTCC1450.00133953449.3098236
CTAAGGT1450.00133953449.3098234
Produced by FastQC (version 0.11.2)