FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_76.3510000003999b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_76.3510000003999b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429000
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTAGG559.572657E-519.0880172
TCTAGAC400.006509694318.7471583
CCCTTAG703.106009E-517.1422581
ACCGATG653.4343015E-416.15148
CACATGG750.001017284113.9978799
CCTTAGA650.006378926313.845671
ACACATG1251.772034E-613.1988
CTATAGT800.001655351613.1230114
GACACAT954.1832664E-412.6296657
AATGCGG954.1832664E-412.62966510
GTATTAG1101.04029954E-412.2722981
TAATACC1101.0414329E-412.2708684
TTCTATA1601.7059756E-712.1856532
CTTATAC1252.5688063E-511.9981813
ATCCTAC1151.5744964E-411.738721
GACAATG1355.636829E-511.1094287
ATACGGT1355.636829E-511.1094286
AAGGGTT950.00599298811.0509585
TATTAGG1253.416694E-410.7983642
ACGGTGT1253.416694E-410.7983648
Produced by FastQC (version 0.11.2)