FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_75.35100000039958.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_75.35100000039958.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3073429
Sequences flagged as poor quality0
Sequence length36
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGCG400.006515611418.7486579
CCGTGCG600.003757615814.9989269
GTATAGG4850.011.446391
CCCTTAG1851.1181892E-610.5433871
ACTGTGC3151.8189894E-1210.4756128
GTGCGAC1356.9667713E-49.99928511
CTGCCTA1200.00295801589.9992849
TATACTG9250.09.8913445
TAAGACC3203.274181E-119.8432064
GCCCACG1250.00414279.60024920
TAGACAG3950.09.4931465
GTCCTAT3502.0008883E-119.4311191
GTAAGAC3051.4206307E-99.3437453
AGGACTG2251.3491444E-69.3328175
TCAGTCC2752.474917E-89.2722158
ACCGTTC1952.3915902E-59.2302588
CAGTCCT2454.3353066E-79.1830169
CTAGTAC3851.4551915E-118.9605433
TATTATA15750.08.8566532
TACCCTG2403.2361731E-68.7495165
Produced by FastQC (version 0.11.2)