FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_74.35100000039914.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_74.35100000039914.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1440099
Sequences flagged as poor quality0
Sequence length36
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTGC1652.5565168E-711.8179368
TTATACT5700.011.3155544
GTATATA6850.011.1719171
GACTCCG1000.00878024510.4997827
CTATCCT2608.967618E-1010.3843984
AGTACTC1451.16712465E-410.3446125
TTAGGAC1602.7758384E-510.3122853
GTATAGA4250.010.2389921
TAAGACT3401.8189894E-1210.1468484
GCATAGT1200.002945804510.00361251
TGGACTG1501.6439777E-49.9997915
TGTACAC1705.4050157E-59.705683
TAAGACG1705.4050157E-59.705684
GTCCTAA1409.6804247E-49.646341
ATCCGCA1250.0041408539.60013429
TCTACGC1250.00414204049.59983
ACACTGC1250.00414204049.59988
CTTATAC3152.5283953E-109.5236113
ATTATAC7700.09.3504553
CTAGCAC1450.00133892929.310153
Produced by FastQC (version 0.11.2)