FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_73.351000000398df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_73.351000000398df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences710418
Sequences flagged as poor quality0
Sequence length36
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG653.4346734E-416.1536185
TAATATA952.6326925E-514.2103264
TTATACA1903.6379788E-1214.2103264
AGTAACG750.001017381813.9998042
TATGGGC800.001655498613.1248163
GTATATG954.1645838E-412.6385171
ATACTAA850.00259983512.3597261
CTAGAAG1601.7090315E-712.1873293
CTCTTAT1252.555968E-512.0065921
GTATAAG1252.555968E-512.0065921
TAGAACT1652.549259E-711.8180154
TTAATAC900.00400492911.6665033
TAGAGGT1059.7039575E-411.4284114
CTTAGGA1059.7039575E-411.4284112
GAATATA1602.2418917E-611.256181
TCTAGAA1202.3420177E-411.2498422
TCTAGAC950.005993415611.0524763
GTATGAG950.005993415611.0524766
TATAGTG950.005993415611.0524765
TCAGTAC1501.3574176E-510.99984553
Produced by FastQC (version 0.11.2)