FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_72.3510000003988f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_72.3510000003988f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94
Sequences flagged as poor quality0
Sequence length36
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATTCCATACGACGTAATCGCCCGCTGAATATTAC11.0638297872340425No Hit
ATTCTAAGCTGTCGAAAATTCGCAACCAATTCAAAA11.0638297872340425No Hit
ACTCAGCGGTGGGATATACTGTGGCAGCGGATAGTG11.0638297872340425No Hit
AAGCAGTATAGATATTAAAGGGACAGTTTTATCGTT11.0638297872340425No Hit
AGCAACACCAACACAGGATCTTGCAGGCTTATGGGT11.0638297872340425No Hit
AGAATACACTCCATTCAAAATTTTGTCGTCACCGCT11.0638297872340425No Hit
GATATGGTCCAAGACTATCAAGGCAAGAACCAATCT11.0638297872340425No Hit
AACAAGAAAGCCTACTCCATTCACAACTCACCATTG11.0638297872340425No Hit
GAGATTGTTTTTACTCAGTTAAAAACTACTGATCCA11.0638297872340425No Hit
GCTATAATATTAGGCATACAAAACATATTAGAAGTT11.0638297872340425No Hit
CCACTACTTTGTATGTGCCCAGCAGCAACAGTAGAG11.0638297872340425No Hit
TCTCTATGCTGTATAACTAAAGATTCATACAGTGGC11.0638297872340425No Hit
CATAGTCTAAAGTACCGATAGCAACCAACATATATA11.0638297872340425No Hit
CTATAGACTGCATTCGAGCAGCCGGAGCATGACAAT11.0638297872340425No Hit
TTTCTAATTTGTGCAATAACTTAACAATATGTACCT11.0638297872340425No Hit
ACAGAAACTCTCGTGTCAAGAAGAGAAAGAAATACC11.0638297872340425No Hit
CTCAATGCAGAGACTGCAGAACAGCTTCCGTTCTTG11.0638297872340425No Hit
TACTAAAAATAGACACGTCATTGGCCAAGCGGCCAA11.0638297872340425No Hit
TCACAATAAAAAACGTGATACAACAAAATAAGAATA11.0638297872340425No Hit
GGTATCCTCTCCTCTCTTGCTCTTTTTTAGTTGAAT11.0638297872340425No Hit
GGTTGAGGCTTTTGATTTTTCTGTAGCATCGGCACC11.0638297872340425No Hit
ATGCAGAAAGCAGCTGATCACATCCAGGACACCAAG11.0638297872340425No Hit
ATAAATAATCGTTCAGTACGACAATGACGATATTCA11.0638297872340425No Hit
GAACTGAATTTCAGGGCTATTACATGTGCGTTTGCA11.0638297872340425No Hit
CAAAAAGTCGAGTCAGACAGTAGATATGAGCAGTAA11.0638297872340425No Hit
CAGTAGAGTAGCCTTGTTGTTACGATCTGATGAGAT11.0638297872340425No Hit
GTACCGGTGACATAATCAGCAGCAGGAGAGAATAAA11.0638297872340425No Hit
TCAGTATTCAATTGTCAGAGGTGAAATTCTTGGATT11.0638297872340425No Hit
GAGCATTGCCCTCTTCATTTTCTATAAAGGAAATGT11.0638297872340425No Hit
TCTAATACTGGTTAATTCTGAGATTCAAAAAATTAT11.0638297872340425No Hit
ACCTCAAGTACCGTAATATGTTCCTTTAGCAGTTGA11.0638297872340425No Hit
CTATTCAAGGTAAGGCGGAGCGAGGTTCACAATCTG11.0638297872340425No Hit
GGGATTGCCCAACTGCTACTACTTGATGGAACGTTG11.0638297872340425No Hit
ATGTATTCTAGATTTTATTCAGTTAAATGGAATGAA11.0638297872340425No Hit
GAAATAACTCTAAGCCTATTATCTTCAATAAAATAA11.0638297872340425No Hit
TTATTTAGGTCCTTCATCTTTTTCACACTGCCTGTG11.0638297872340425No Hit
CCCAAACAATAGCCACAATGATCAACGTAACATTAA11.0638297872340425No Hit
AACAGAACAGAGATAATTTGTTGTAATAATTGTGCG11.0638297872340425No Hit
CCAATAGGCACAATAAATAAATTAATTGATGATTTG11.0638297872340425No Hit
CCAGTACACCAGTACACCAGTACACCAGTACAAAAG11.0638297872340425No Hit
GGTGAAATGGCATCGTCATTGATTGCCGAGGACAGA11.0638297872340425No Hit
ATTATAAATGGTTATGCCAACTTCAATAAGTTTGAT11.0638297872340425No Hit
TAGTACACCTGGAATTCAAATAGCACGCGCTAAGAA11.0638297872340425No Hit
CCGATAGTAGTCCCAATTCCAGAAGCATATAGGTGA11.0638297872340425No Hit
GTTTAAGTCTTCATCGATAGTGAGTGCGTCGTAATC11.0638297872340425No Hit
CAAAGAAGATGGATTATTACCTTGGAGAGCATAATG11.0638297872340425No Hit
CTGTTGCCGTCATCAGGTCTCTTTTCTGTGTTCCCA11.0638297872340425No Hit
CCAATCTAATGCATATAATACAAAAACTAATATGTA11.0638297872340425No Hit
AGTAAAGGAAGAGAGACAGAGATGGAAAAAGAAAAT11.0638297872340425No Hit
GTCTTTACCCTCACTATTACTTGATAGTCCGTACAT11.0638297872340425No Hit
ACTCTCTTTGATCCAACCGTCAGAAAGTCGTAAATT11.0638297872340425No Hit
GGTAAAACCCCCGTTATATCATTTCCGTGGGTCCTC11.0638297872340425No Hit
CTCGACATCAAGCTCAACATTCTTGGGTCCCTTGTA11.0638297872340425No Hit
TCTTGAGACAATTGAAGACATATGACTCCATCATCT11.0638297872340425No Hit
GTCTCGTATTCCACGAAAGCTAGGTTACGAACGCTA11.0638297872340425No Hit
TCGTAACCCACGGCATTGAAGCGCTGTTGAAGTTTG11.0638297872340425No Hit
CGCCAATGGAGATGTTGACCGCATGACGGTTAATCT11.0638297872340425No Hit
AATCAACGTTACTGACACAGGTAACGTTTCATATGA11.0638297872340425No Hit
CAAGATTACATTGAATTACAACGATCTGGAATGAAG11.0638297872340425No Hit
CCCGAATACTTCCTGATTGATATTGGTCTCCGTAGT11.0638297872340425No Hit
AAGTTCAAAAGTGTCTTGTATCATATACTAAAGGAA11.0638297872340425No Hit
AAATGATACCTGGCCTATCGTATCAGGAAAAGAAAA11.0638297872340425No Hit
AAATAAGATTGTCCACCGGGATATGAAGGCAGCAAA11.0638297872340425No Hit
TTATAAATTAGCGTAATCACTCGATGAACAGAATTA11.0638297872340425No Hit
CAATTAGCAGCGCATTTTGTTTATTCAATGCTAGTC11.0638297872340425No Hit
AAATTCAAGCAACATAATCCAACACTAGACCTAATA11.0638297872340425No Hit
AAATATCAAATATCGAAGAAATACACCAAATATTCA11.0638297872340425No Hit
AACAAAGTACCACACAATGAGTGATAATACTTAAAA11.0638297872340425No Hit
CCAGCATCCCTTGCCATCCCGTCTGACATATTTTTC11.0638297872340425No Hit
CATTAGTGCCGTCGCCAGTGGAAAAATGGCGTTAGC11.0638297872340425No Hit
GCTAAGTGAAAGCCCTTCACTATCAACCCATCTTTG11.0638297872340425No Hit
CCAATACTCTCTCTTCTAATCTATAAACATCCAATA11.0638297872340425No Hit
ATCCCTGCCTTCGGCTAATTTTAAACCAACTTTTTA11.0638297872340425No Hit
CATCTGGACCGTTCCAACAAGTATGTGCGGATCCTT11.0638297872340425No Hit
AATTATACCACCGTCAAGATAGAACATCCATGGGAC11.0638297872340425No Hit
ATTTTCACCAACAACGCCAGCGCTCAAAAAGCTATT11.0638297872340425No Hit
GAATACAACCAACACCAGCAGTAAACAACAACACAC11.0638297872340425No Hit
GAGGTGATCAATGCGAGGTGGTTGGCCAAGGGCAAG11.0638297872340425No Hit
ATTATCCACTTCGACGCTACACCAATCTACTAGGAT11.0638297872340425No Hit
GGCCATTCCTCAAATGACAAGAACTTCATTAAAAAA11.0638297872340425No Hit
AATAAGATAAGGATTAAAAAAATCATAGGAAAGAAA11.0638297872340425No Hit
ATAATAGTTTCTATACTCATGGAAAGTGCAACCGAT11.0638297872340425No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAG11.0638297872340425No Hit
CAAAGAAAAGAACAAGAGCAGCCAACAACCCATACT11.0638297872340425No Hit
ACTGTAAACAGATCTTATATTCTCCAGATTCCAAAG11.0638297872340425No Hit
TCACTGGGAGTTCCTAAATTTACCACGAGAGATAGA11.0638297872340425No Hit
GGCAAATGCAAATATGTGCGCTTGGTAACAGTTATG11.0638297872340425No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGA11.0638297872340425No Hit
CAATTAGACAAGGAACCAGTCCAAAAAGTACGTGCA11.0638297872340425No Hit
AATAAACTCTTCAAGGGTAAGCTTGTCTTGAGACTT11.0638297872340425No Hit
TACCATGAAATACGCCCACACCAACTTCACAACACG11.0638297872340425No Hit
AAATATGGCAGATAGGCTCTTACCTGCATAATTAAC11.0638297872340425No Hit
CAACAAAACCATGGCCTTGGAATCTGTAGCCTCTAA11.0638297872340425No Hit
TCTCATTCATCGCTTGATTTCCGGCCTGCAAAAATA11.0638297872340425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCATT50.029.99999824
TTCATTA50.029.99999825
CAAGAAC50.029.99999818
TCCTCAA50.029.9999987
ATGACAA50.029.99999814
TAAAAAA50.029.99999830
TGACAAG50.029.99999815
CATTCCT50.029.9999984
AGAACTT50.029.99999820
ACTTCAT50.029.99999823
AACTTCA50.029.99999822
TCAAATG50.029.99999810
TTCCTCA50.029.9999986
CATTAAA50.029.99999827
GAACTTC50.029.99999821
GACAAGA50.029.99999816
ATTAAAA50.029.99999828
CCATTCC50.029.9999983
GGCCATT50.029.9999981
CCTCAAA50.029.9999988
Produced by FastQC (version 0.11.2)