FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_70.351000000397fa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_70.351000000397fa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences560642
Sequences flagged as poor quality0
Sequence length36
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAAA400.00650989618.7482722
TACTAAA601.8648407E-417.4983862
TCTATAC706.0342415E-414.9986183
ATACAGC1156.932569E-714.3465036
CTCTTAT750.00101682613.9999581
CCTTATG650.00638079713.8461131
TACCGAG650.006384568313.8448787
TACACTG1004.2665648E-513.4987555
TATAGAC902.650218E-413.3321053
TGTACCG954.1847292E-412.6304155
ACCGCTC850.002611187812.3518028
AACAGCT850.002611187812.3518027
CAGACCT850.002611187812.3518024
TGTACAC1151.5769899E-411.7380493
TACCGTC1151.5769899E-411.7380497
ATATTAT900.004002795611.6666331
TCATATA1059.7047223E-411.4275182
GATATAG1459.306823E-611.3792761
AGACTGA1202.3420385E-411.2489636
CCCCAAT1100.001430733510.9090591
Produced by FastQC (version 0.11.2)