FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_67.35100000039730.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_67.35100000039730.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830852
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACT802.4972724E-718.7480814
TGTACCG908.5845386E-716.6649635
TACACTC653.4378594E-416.1521955
TACCGCT653.4378594E-416.1521957
CTAGGAC1054.270849E-614.2842543
TACACCG952.635945E-514.2090715
ATAGGGG750.001018315813.9985693
AGTGTGT750.001018315813.9985698
GTACACA650.006375688613.8489051
TGTCCGG650.00638841213.84473810
TGCACTG902.6528558E-413.331975
GTCTTAG800.001653259513.1276081
TTAGGCT1056.7518515E-512.8558284
TACACTG1404.5068919E-712.8558275
TATACTG3900.012.69100955
AGAGTGC954.1888637E-412.6302866
TTTACAC1201.693699E-512.4987213
TACCGAC850.002613215512.3516777
CCTAAGA1252.5743178E-511.9987732
ACTCTGC1151.5788928E-411.7379298
Produced by FastQC (version 0.11.2)