FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_65.351000000396b7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_65.351000000396b7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1848921
Sequences flagged as poor quality0
Sequence length36
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGTAC1753.075911E-912.8565863
ACCGCGT850.00261467712.3520748
TCTTATA3650.011.095712
GTATTAA2402.9889634E-810.0028141
TAGACAG2251.2555392E-79.9995675
ATACTGT3600.09.9995676
TTAGGCT1250.00414374849.5995854
TACACAG3001.0513759E-99.4995895
TCTAGAC2557.665221E-89.4113573
GACCGTA1450.00133958079.3099417
TAGGCAG1450.00133958079.3099415
GTTCTAA2105.644766E-69.2883271
GTATTAT3451.4551915E-109.1330031
GTCTAGG1654.2711015E-49.0934661
CTTATAC4150.09.0357543
GTCTATA3501.9463187E-109.0025321
GTCTTAA1500.00181913689.0025321
TAAAGCG2003.220483E-58.9996115
GGACCGT2003.220483E-58.9996116
CATACAC4055.456968E-128.8885043
Produced by FastQC (version 0.11.2)