FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_64.35100000039674.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_64.35100000039674.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187713
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCATG550.002102743616.362429
CCGTCGC1201.1232933E-613.7489779
ACCGTCG1551.133576E-712.5797098
AGCGTCC850.00261380712.3520238
CCCTTAA1652.544275E-711.8217811
CCCTAAT900.00399810911.670221
CTACCCT900.004009445711.6657994
GAACTGC1303.8466613E-511.5376036
AGACCTC1059.7172597E-411.4277215
TATTAGG3500.011.1420292
ACTGGTC1355.651876E-511.1102858
GGCCAGT950.006000126211.0518096
GTGTGCT1100.001433632910.9082799
GTCGTTC1100.001433632910.9082798
TACACCG1653.2737516E-610.9082795
TATGGCG1551.9556519E-510.6443693
CACGCCT1000.00877125310.50098724
CATGCGC1000.0087822110.4992210
GTCCTAA1304.900798E-410.3877781
GTGTATG1809.2100945E-610.0030461
Produced by FastQC (version 0.11.2)