FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_63.35100000039631.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_63.35100000039631.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1814557
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG900.00400987911.6661749
GGGGTCG900.00400987911.66617414
GTATTAG4100.011.3431721
TAGACGG1202.3458505E-411.2498355
GGACAGC950.00599952611.0524696
CTAATAC4550.010.8789623
TAGGAGT2402.6539055E-910.6248444
CTAGGAC1150.002075999310.43462853
ACAGCGT2301.5512342E-810.4343418
CCCTAAG1304.910437E-410.386181
GCCGCGT1304.91543E-410.385035525
CGATCAC1451.1667831E-410.34524524
AGACTGT3253.6379788E-1210.1536976
TAGACTG1952.2026907E-69.9998535
CTAGGGC1200.0029556259.9998534
CAGGACT2701.7262209E-99.9998524
ACCGTGG1200.00295639.99957758
GTAGACA2609.888026E-99.8075484
ACTATGC4900.09.7955058
CTAGACT1705.4067517E-59.7057394
Produced by FastQC (version 0.11.2)