FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_62.351000000395fc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_62.351000000395fc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1043700
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG2300.013.6979931
GTCTTAG1302.7524165E-612.6944771
TAGACTG954.189271E-412.6307125
ATAGGCT850.002613329612.3520934
CTTACAC1101.0433897E-412.2718843
CTAATAC3550.012.252683
CTGTGCA1151.5798458E-411.73776159
CTGCGCA900.00401015311.6653079
TATTAGG2452.5465852E-1111.6318532
TCTTATA2605.456968E-1211.5376692
CCTAATA3650.011.5060592
CTAGGAC1059.7150676E-411.4277873
TAGGTAG1901.3242243E-711.0518725
ACTATGC2053.2043317E-810.974338
GGCTTAG1100.001430449210.9109551
TTAGGTA2504.5292836E-1010.7992594
ATGGACC1408.177207E-510.7135514
TCTTAGG1000.008780641510.4992792
CGTCGCT1451.1675519E-410.34362210
TCTATAC1756.6120683E-610.2850083
Produced by FastQC (version 0.11.2)