FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_61.351000000395b8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_61.351000000395b8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1460491
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG1302.7617425E-612.6915255
CTACCCC954.1908023E-412.63084
TATCTCG1406.3215175E-611.7849885
CCGAGCC1059.718557E-411.42786716
GTCTTAC1202.3388331E-411.25315951
CACGTCC950.006000673411.0519510
ACCGTGG1100.001433822210.9084198
TTAGGTA3200.010.7805854
TAGGTAG2104.5696652E-810.7136255
TATACAG7050.010.6376425
CTCCTAT1704.6623736E-610.5912081
GCTCGAT1000.00877408710.50079225
TCTAGGC1000.00878299910.4993523
CATACAC3009.094947E-1210.4993523
TACAGCG2002.6890302E-710.4993527
GCCTAAT1150.002070487510.4377131
GTCCCCA1304.918823E-410.38397511
TATGTAC3650.010.2733393
CCTAATA3950.010.2525332
CTATACA5050.010.0983884
Produced by FastQC (version 0.11.2)