FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_60.35100000039575.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_60.35100000039575.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658749
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC455.465487E-419.9987353
TAGACAG805.1694606E-616.8739325
ACTAGAC650.006384909613.8452783
AACCACT1201.6904414E-512.50015819
AATACCG1856.9994712E-912.1613935
ACAGGGC1006.4459554E-411.9992418
ACTGTGT1006.4459554E-411.9992418
TATACTG2800.011.7849685
TATACTC1703.754758E-711.4698635
CCTATAC1059.706312E-411.4278493
CTTATAC1202.3425937E-411.24928953
TTACACA1755.462607E-711.1421534
CTGACCT950.005994823311.0519324
CTAGTAG950.005994823311.0519323
AACTCTC950.005994823311.0519327
CTGTGCA950.005994823311.0519329
AAGAGGC1501.3577419E-510.9993047
GTATTAG2251.0881195E-810.6708521
TCTTATA2701.5279511E-1010.5548882
TCTAGGA1000.00877451710.4993362
Produced by FastQC (version 0.11.2)