FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_59.35100000039532.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_59.35100000039532.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123300
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGGG500.001110478217.99933215
CGGTCGG550.00210212416.3630313
TCGGGGG600.003754777814.99944316
ACGTCGC650.00638373413.84687224
GGTCTCG800.00165682913.12451415
TACGACT1302.7607512E-612.6912724
GTAGGAC850.002613797812.35193353
TAGGCTG850.002613797812.35193355
TAACTAG850.002613797812.35193355
CTAATAC2800.012.3204233
TAGTACC2301.05501385E-1011.7381724
TGTACCG900.00400943111.6657155
GTCTTAG1355.6414196E-511.1121831
CCTTACG950.006000104411.051732
CTAGGTG950.006000104411.051733
TAATACT3950.011.011764
AGTGCGC1100.001433614210.9082018
GACTAGA1000.00877059710.5010131
CCATCCC1000.00878218210.4991449
GTAGTGC1000.00878218210.4991448
Produced by FastQC (version 0.11.2)