FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_58.351000000394fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_58.351000000394fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708971
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCCC550.002100904516.363258
CATAACC755.617115E-515.9996244
GTGTATC600.003747339415.0028211
CCCTTAA600.003747339415.0028211
CCTACAC600.003752618814.9996463
AACGTCG650.00638386113.8458285
GGACTTC650.00638386113.8458286
TACACAG902.6502286E-413.333025
GACAGTC800.001655610413.1246937
CTAATAC2650.013.0185623
ACACGAT850.002611019612.3526516
CTTACAC850.002611019612.3526513
TAACACT1354.202455E-612.2219354
GTATTAG1252.5654246E-512.0022571
GTACTAA1406.2911186E-611.7879311
GGCTATA1151.5740062E-411.7413391
TAGGATC900.004005199311.6663924
GACTGGT900.004005199311.6663927
CTATGCA1951.5266778E-811.538199
GTATAAT3000.011.5021641
Produced by FastQC (version 0.11.2)