FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_57.351000000394b9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_57.351000000394b9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507230
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGC550.002100387616.3625626
AGAACCG550.002100387616.3625625
ACAGTGT653.4336024E-416.1527848
GGTAGTC653.4336024E-416.1527847
TAGACAG755.6140885E-515.9989495
CCTTAGG600.003749251415.0004931
TAGTCAG650.006382344313.8452439
GACCGTC800.001655005713.1241387
TAGAACT800.001655005713.1241384
TATATCG954.1825752E-412.630755
GTATTAG1456.86754E-712.4142021
TAAGGGA850.002610078312.3521294
TCACACA1006.4362475E-412.00039530
TATTAGG1501.0360036E-611.9992122
TCTTATG900.00400377511.66592
GGACAGT900.00400377511.66596
GTATAAT1703.743553E-711.4709641
TAGGAGT1059.699784E-411.427824
CTATGGA1355.6366018E-511.1103812
TTAGGAC950.00599171311.0519063
Produced by FastQC (version 0.11.2)