FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_56.35100000039476.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_56.35100000039476.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences761120
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCGG400.0065098418.74934410
TAGACTG1001.4214311E-716.4994225
GCATGCG550.002101258416.3630629
TCTAAGC951.5079222E-615.7889193
GGCTAAC600.00374013915.0073611
GCGAACC600.003751603715.00046129
GAGTGGG600.003753243714.9994757
CTTATAC1900.014.9994743
GTCTAAG952.6206762E-514.2175011
TCCTAGA952.6320691E-514.2109612
ATCCTAG851.6255108E-414.1245771
GCCTCAC750.001017660613.9995120
TAGAGTG1352.8933937E-713.3328675
CCAGAGC800.001655949713.124543
AGGCCTC800.001655949713.1245418
GTATAAG2750.013.0973341
GGACTGC1056.7461515E-512.8566936
TAGTACT1651.8600986E-812.7268274
CTAGTAC1201.692087E-512.4995623
AGTACTG2307.2759576E-1212.390875
Produced by FastQC (version 0.11.2)