FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_55.35100000039433.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_55.35100000039433.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1037939
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT550.002101594116.3634830
GTCCTAA1206.825212E-815.0078081
GCACGAC706.0388097E-414.99913213
TAGGTAG1156.942555E-714.3476885
TAGACGG750.001017902713.9998655
CTGAGCC650.00638794413.8453549
ATCACAC2250.013.33320529
CTAATAC3750.013.1998733
TAATACT5400.013.055434
TCTAGGG954.18715E-412.6314582
CTATGCA2850.012.6308499
TGGGGCT954.188852E-412.63084917
TAGGACT1201.6930479E-512.4998794
GCCTAGA850.002601789512.3593721
AATACTA4050.012.2221045
ACTGAGC1354.2093725E-612.2215168
TGAGGCC1354.2093725E-612.22151616
CTAAGCA1601.7118873E-712.1873834
TAGACTG1501.0387776E-611.9998855
TCTAAGC1754.2302418E-811.9998843
Produced by FastQC (version 0.11.2)