FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_54.351000000393fe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_54.351000000393fe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336503
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGCC800.001656917513.12472257
AGACGGC1056.7527006E-512.8568716
GTGGTCC1056.7574365E-512.8559089
TCTATAC3450.012.6084283
GTCGGGT850.002614528612.35175516
TCTAGGG1406.317541E-611.7854653
CCTAGGA900.004008302511.6664192
GGTCGGG900.00401054411.66554615
CTAATAC3450.010.8693343
GTCCTAA1807.8605444E-710.8347241
TTCTAGG2504.5292836E-1010.7997712
TATACTG4600.010.7606415
CTAACCT2104.5654815E-810.7140594
TATACCG2104.5654815E-810.7140595
ATACTGT3550.010.5631566
ACAGCCC1000.00878458810.4989928
GCTGTGC1150.002076787410.4337818
CTGTGCA1150.002076787410.4337819
CTAGCCT1304.9164053E-410.3843964
ACTGTGC1304.9197976E-410.3836188
Produced by FastQC (version 0.11.2)